Alkaline Phosphatase Staining Kit II 堿性磷酸酶染色試劑盒

【溫馨提示I】：見(jiàn)堿性磷酸酶產(chǎn)品專(zhuān)題，了解更多相關(guān)產(chǎn)品資料；

【溫馨提示II】：見(jiàn)堿性磷酸酶活性檢測試劑盒產(chǎn)品；

【溫馨提示III】：見(jiàn)堿性磷酸酶常用生化底物及試劑盒產(chǎn)品；

基本介紹（Stemgent，00-0055）：

處于未分化狀態(tài)的胚胎干細胞（ES）和誘導多潛能干細胞（iPS）典型特征為具高水平的堿性磷酸酶表達，同時(shí)結合其他的一些表面標志物，來(lái)顯示未分化細胞具自我更新的潛能。堿性磷酸酶（ALP，或AP）是一種水解酶，堿性條件下能夠將核苷酸、蛋白、甾醇等分子去磷酸化作用。

The undifferentiated state of embryonic stem (ES) and induced pluripotent stem (iPS) cells can be characterized by a high level of alkaline phosphatase (AP) expression which, along with the expression of other surface markers, indicates undifferentiated cells with the potential to self-renew.AP is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. When fixed ES or iPS cells are stained using the AP Staining Kit, undifferentiated cells appear red or purple, whereas differentiated cells appear colorless.

結果呈現：

固定ES或iPS用堿性磷酸酶染色試劑盒（Stemgent，00-0055）染色后，未分化細胞呈紅色或紫色，而分化細胞無(wú)色。

產(chǎn)品特性：

1. 試劑盒組分

1. 2. 保存和穩定性

4°C保存，不可凍融。按照指示保存收到貨后至少能存放6個(gè)月。

1. 3. 質(zhì)量控制

本堿性磷酸酶染色試劑盒以人和小鼠干細胞為對象進(jìn)行功能測試，以確保產(chǎn)品質(zhì)量。（The AP Staining Kit II is functionally tested on human and mouse ES cells to ensure product quality.）

訂購信息：Stemgent原裝進(jìn)口，干細胞研究專(zhuān)家精品，品質(zhì)認證，歡迎選購。

00-0055 Datasheet

00-0055 Staining Protocol

相關(guān)信息：自產(chǎn)堿性磷酸酶染色試劑盒，在保證優(yōu)秀質(zhì)量的情況下，提供更有競爭力的價(jià)格，歡迎選購。

參考文獻：

Pease, S., Braghetta, P., Gearing, D., Grail, D., and Williams, R.L. (1990) Isolation of embryonic stem (ES) cells in media supplemented with recombinant leukemia inhibitory factor (LIF). Dev Biol. 141: 344-352.

Smith, A.G., Nichols, J., Robertson, M., and Rathjen, P.D. (1992) Differentiation inhibiting activity (DIA/LIF) and mouse development. Dev. Biol. 151: 339-351.

Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.

Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.

Additional Publications

Momcilovic O; Sivapatham R; Oron TR; Meyer M; Mooney S; Rao MA; Zeng X. "Derivation, characteriazation, and neural differentiation of integration-free induced pluripotent stem cell lines from Parkinson's disease patients cfarrying SNCA, LRRK2, PARK2, and GBA mutations." PLoS ONE 11(5): e0154890. doi:10.1371/journal.pone.0154890 (2016)

 — — Written/Edited by V. Shallan【版權歸MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科學(xué)和生物技術(shù)領(lǐng)域研究的試劑、儀器和實(shí)驗室消耗品與實(shí)驗服務(wù)工作，主要從事細胞生物學(xué)、植物學(xué)、分子生物學(xué)、免疫學(xué)、生物化學(xué)、蛋白組學(xué)。生物制藥與診斷試劑研發(fā)生產(chǎn)等領(lǐng)域。 本公司秉承“以人為本，以誠為信、合同守信”的經(jīng)營(yíng)理念。堅持"品質(zhì)保障"的原則為廣大客戶(hù)提供優(yōu)質(zhì)產(chǎn)品。