Stemfactor? Activin A, Human Recombinant 重組人激活素A

產品關鍵詞：

na?ve stem cells 初始干細胞；neural progenitor cells 神經祖細胞；WH-4-023；CHIR99021；Stemfactor? LIF；

產品描述：

Stemfactor? Activin A是一種結晶級別的重組蛋白制備物，提供非常高的純度。激活素A（Activin A）具有雙重功能，不僅能維持干細胞潛能也能促進分化。成熟的重組人激活素A是2條116個氨基酸殘基組成的βA亞基通過二硫鍵連接的二聚體，分子量約25kDa。

Stemgent公司提供支持初始潛能干細胞應用需要的5i/L/A培養基添加劑（5i/L/A media supplment）所有成分，如下：

產品特性

1. 來源：E.Coli

2. 配方：Lyophilized from 20% acetonitrile

3. 氨基酸序列：GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI IKKDIQNMIV EECGCS

4. Uniprot序列號：P08476, residues 311-426

5. 純度：＞98%（SDS-PAGE）

6. 內毒素水平：Less than 1.0 EU/μg of Activin A as determined by the LAL method.

7. 生物活性：The ED50 is less than 6 ng/ml as determined by its ability to inhibit the proliferation of mouse MPC-11 cells.

8. 重溶：Centrifuge briefly and reconstitute Activin A in 10 mM HCl before dilution in cell culture medium. Avoid freeze-thaw cycles as it can result in loss of activity.

9. 保存和穩定性：Activin A is shipped at room temperature. Lyophilized Activin A is stable for up to 6 months from date of receipt when stored at -20°C to -80°C.  Reconstituted Activin A, at concentrations greater than 0.1 mg/ml, is stable for up to 3 months when stored at -20°C and for 1 year when stored at -80°C

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引用文獻：

• Villa-Diaz LG; Kim JK; Laperle A; Palecek SP; Kresbach PH. "Inhibition of focal adhesion kinase signaling by integrin a6b1 supports human stem cell self-renewal." Stem Cells doi: 10.1002/stem.2349 (2016)

• Zhu S; Russ HA; Wang X; Zhang M; Ma T; Xu T; Tang S; Hebrok M; Deng S. "Human pancreatic beta-like cells converted from fibroblasts." Nature Commun 7:10080 (2016)

• Saxena S; Ronn RE; Guibentif C; Moragheba R; Woods N-B. "Cyclic AMP signaling through Epac axis modulates human hemogenic endothelium and enhances hematopoeitic cell generation." Stem Cell Rep doi:10.1016/j.stemcr.2016.03.006 (2016)

• Mora-Castilla S; To C; Vaezeslami S; Morey R; Srinivasan S; Dumdie JN; Cook-Andersen H; Jenkins J; Laurent LC. "Miniaturization technologies for efficient single-cell library preparation for next-generation sequencing." J Lab Automation 2016:1 (2016)

• Qian X. "Defining the mechanism by which synthetic polymer surfaces support human pluripotent stem cell self-renewal." PhD Thesis, University of Michigan (2015)

• Villa-Diaz LG; Kim JK; Lahann J; Kresbach PH. "Derivation and long-term culture of transfene-free human induced pluripotent stem cells on synthetic substates." Stem Cells Trans Med 3:1410 (2014)

• Bukong, T.N., Lo, T., Szabo, G., and Dolganiuc, A. (2012) Novel developmental biology-based protocol of embryonic stem cell differentiation to morphologically sound and functional yet immature hepatocytes. Liver Int. doi: 10.1111/j.1478-3231.2011.02743.x. [Epub ahead of print]

• Kunisada, Y., Tsubooka-Yamazoe, N., Shoji, M., and Hosoya, M. (2012) Small molecules induce efficient differentiation into insulin-producing cells from human induced pluripotent stem cells. Stem Cell Res 8: 274-284.

• McCracken, K.W., Howell, J.C., Wells, J.M., and Spence, J.R. (2011) Generating human intestinal tissue from pluripotent stem cells in vitro. Nat Protoc 6: 1920-1928.

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  上海懋康生物科技有限公司是一家涉足于生命科學和生物技術領域研究的試劑、儀器和實驗室消耗品與實驗服務工作，主要從事細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發生產等領域。 本公司秉承“以人為本，以誠為信、合同守信”的經營理念。堅持"品質保障"的原則為廣大客戶提供優質產品。

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