alamarBlue® protocol: Method to determine LD₅₀ using alamarBlue

alamarBlue can be used to determine the median lethal dose LD50 value and similar assays testing the effects of a toxin, radiation, or pathogen against cell cultures.

Use semi-log graph paper to plot the percent of untreated control (using equation 5) for each dilution of a given test agent on the y-axis vs. the concentration of the test agent on the x-axis. 

To determine the LD₅₀ endpoint from the graph, read from where the 50 percent point intercepts the Dose. 

Response Curve to the concentration along the x-axis. This concentration is the LD₅₀ value. 

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