【務(wù)必注意】：初次使用離子探針的用戶(hù)，強烈建議配合：Pluronic F-127, Cell Culture Tested 細胞培養級（MS4301-1G）一起使用，以提高探針的水溶性和胞內加載性。 

產(chǎn)品標簽

PBFI AM 鉀離子指示劑/探，SBFI AM 鈉離子指示劑/探針，Asante Potassium Green-2，Valinomycin 纈氨霉素，CAS NO：124549-23-1

產(chǎn)品信息: 現貨供應，歡迎咨詢(xún)。

產(chǎn)品描述

PBFI，英文全名Potassium-binding Benzofuran Isophthalate，一種K+敏感的熒光探針，用來(lái)測定細胞和細胞內區隔（Intracellular compartments）的K+水平變化。雖然PBFI對K+的選擇能力弱于Ca2+指示劑比如Fura-2，但在其他一價(jià)陽(yáng)離子存在體系中，PBFI足以檢測K+的生理濃度。結合離子后的PBFI光譜變化可通過(guò)激發(fā)光比率測定來(lái)分析，其能與使用相同光濾片和儀器檢測的探針Fura-2共同使用。

PBFI對K+的解離常數（Kd）非常依賴(lài)于Na+的存在與否。在不含Na+的體系，PBFI對K+的Kd值為5.1mM；而在含135mM K+/ Na+總濃度（約為生理離子強度）的溶液體系，PBFI對K+的Kd值為44mM；若緩沖液中的Na+用四甲基氯化銨所替代，PBFI對K+的Kd值變?yōu)?1mM。氯化膽堿和N-甲基葡萄糖胺是培養基中另兩種可能替代Na+的化合物。雖然PBFI對K+的選擇性比Na+僅強1.5倍，這一選擇能力已足以滿(mǎn)足檢測需求，因為正常情況細胞內K+濃度比Na+高10倍左右。

本品為乙酰氧基甲基酯（Acetoxymethyl ester, AM ester）形式的PBFI，CAS NO：124549-23-1，具有細胞膜滲透性，只需簡(jiǎn)單孵育即可進(jìn)入細胞，常用加載濃度范圍5-10μM，加載時(shí)間40min-4h，根據具體的實(shí)驗要求和細胞類(lèi)型來(lái)調整。

產(chǎn)品特性

1） 化學(xué)名：4,4'-[1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diylbis(5-methoxy-6,2-benzofurandiyl)]bis- 1,3-benzenedicarboxylic acid, tetrakis[(acetyloxy)methyl] ester

2） 同義名：Potassium-binding Benzofuran Isophthalate Acetoxymethyl ester

3） CAS NO：124549-23-1

4） 分子式：C58H62N2O24

5） 分子量：1171.1

6） 純度：≥95%

7） Ex/Em：~340,380/500 nm

8） 外觀(guān)：黃色至橙色粉末

9） 溶解性：溶于DMSO（10mM）和甲醇

10）化學(xué)結構式：

保存與運輸方法

保存：-20oC避光干燥保存，2年有效。

運輸：冰袋運輸。

注意事項

1） PBFI AM易受潮，粉末需干燥保存；粉末需用無(wú)水DMSO溶解，配制儲存液（如10mM），置于-20℃干燥避光保存，小量分裝避免反復凍融，至少3個(gè)月穩定。

2） PBFI AM由于水溶性較差，建議使用Pluronic F-127以?xún)?yōu)化探針的細胞加載效率。通常情況，將PBFI AM的DMSO儲存液與等體積Pluronic F-127（25% w/v）混合均勻，之后即刻加入適量的細胞加載緩沖液（cell loading buffer）中達到所需濃度。

3） 為了您的安全和健康，請穿實(shí)驗服并戴一次性手套操作。

 — —Written/Edited by V. Shallan【版權歸MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科學(xué)和生物技術(shù)領(lǐng)域研究的試劑、儀器和實(shí)驗室消耗品與實(shí)驗服務(wù)工作，主要從事細胞生物學(xué)、植物學(xué)、分子生物學(xué)、免疫學(xué)、生物化學(xué)、蛋白組學(xué)。生物制藥與診斷試劑研發(fā)生產(chǎn)等領(lǐng)域。 本公司秉承“以人為本，以誠為信、合同守信”的經(jīng)營(yíng)理念。堅持"品質(zhì)保障"的原則為廣大客戶(hù)提供優(yōu)質(zhì)產(chǎn)品。

文獻引用：

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Tong W et al. Phthalocyanine functionalized poly(glycidyl methacrylate) nano-assemblies for photodynamic inactivation of bacteria. Biomater. Sci., 2019,7, 1905-1918

Sodium-binding benzofuran isophthalate acetoxymethyl ester (SBFI-AM) and Potassium-binding Benzofuran Isophthalate Acetoxymethyl ester (PBFI-AM) were purchased from MKbio. China.

[2]

Li R et al. Biofilm inhibition and mode of action of epigallocatechin gallate against Vibrio mimicus. Food Control, Volume 113, July 2020, 107148

Then PBFI probe (ShangHai MaoKang Bio technology Co., LTD., China) was added and incubated at 37 °C for 90 min. The cells were washed, collected and resuspended with PBS buffer. Aliquots (100 μL) of bacterial suspension were transferred to a Corning 96 well black plate, and 100 μL of various concentrations of EGCG were dispensed in the microtiter plate wells.

[3]

Liu Y, Zhen W, Wang Y, Song S, Zhang H. Na2S2O8 Nanoparticles Trigger Antitumor Immunotherapy through Reactive Oxygen Species Storm and Surge of Tumor Osmolarity. J Am Chem Soc. 2020 Dec 30;142(52):21751-21757. doi: 10.1021/jacs.0c09482. Epub 2020 Dec 18. PMID: 33337859.

4T1 cells were inoculated into glass bottom culture dishes for 24 h. Then, adding PNSO NPs medium solution (80 μg/mL) to continue co-culture for 4 h. The treated 4T1 cells were further incubated with 10

μM Na+ indicator SBFI AM (sodiumbinding benzofuran isophthalate acetoxymethyl ester, MKBio, 90%) in 0.04% Pluronic F-127 (MKBio) and the fluorescence signal was measured by CLSM.

[4] 

Liang Z, Yang Y, Yu G, et al. Engineering aluminum hydroxyphosphate nanoparticles with well-controlled surface property to enhance humoral immune responses as vaccine adjuvants. Biomaterials. 2021 Jun;275:120960. DOI: 10.1016/j.biomaterials.2021.120960.

[5] BMDMs were treated with AAHPs (250 μg/mL) in the presence of LPS at 500 ng/mL for 5 h. Then PBFI AM (Mkbio, Shanghai, China) was added to the cells at the concentration of 10 μM, and cells were incubated at 37 °C for 1h. Triton X-100 (0.2%) treated cells were used as controls. The fluorescence of PBFI AM was measured at the Ex/Em of 340/615 nm. The data were expressed as relative fluorescence intensity (RFI) defined as the fluorescence intensity of AAHPs-treated BMDMs normalized to the intensity of control cells.

[6] Jia Y, Yang B, Shi J, Fang D, Wang Z, Liu Y. Melatonin prevents conjugative transfer of plasmid-mediated antibiotic resistance genes by disrupting proton motive force. Pharmacol Res. 2022 Jan;175:105978. doi: 10.1016/j.phrs.2021.105978. Epub 2021 Nov 21. PMID: 34813930.

As for the detection of intracellular K + concentration, the PBFI-AM (K + indicator) fluorescence dye (Maokangbio, Shanghai, China) labeled cells (10 μM) in the presence of…..